In-vitro-antidiabetic Activity of N-butanol Extract of Sesamum Indicum

Objective: The present study was aimed to investigate the inhibitory effect of n-Butanol extract of black sesame seeds on the enzymes αamylase and αglucosidase followed by its Total antioxidant capacity. Methods: The Black sesame seeds were extracted in n-Butanol, powdered and stored for future studies. Phytochemical analysis was performed to detect the presence of many phytochemicals. The extracts were screened Alpha-amylase inhibition assay and Alpha-glucosidase inhibition assay. The total antioxidant capacity of the n-Butanolic extract was evaluated by the phosphomolybdenum method. Results: The obtained results showed the high alpha-glucosidase inhibitory affect than the alpha-amylase inhibition when compared to standard acarbose. Even the Total antioxidant capacity was equivalent when compared with the standard Ascorbic acid values. Conclusions: Based on the results, it can be concluded that Sesamum indicum herb has potential phyochemicals which can be used to reduce the postprandial hyperglycemia by inhibiting carbohydrates metabolizing enzymes αamylase and αglucosidase, and also to combat the free radicals by its anti-oxidant activity.